Y13-259细胞

Y13-259细胞

生长状态： 悬浮生长

数量： 大量

运输方式： 冻存运输

是否是肿瘤细胞： 0

物种来源： 褐鼠

ATCC Number： CRL-1742™

细胞形态： **样

**类型： IgG1

Designations: Y13-259

Y13-259细胞Depositors: JR Stephenson, ME Furth

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Rattus norvegicus (myeloma) deposited as rat (B cell); rat (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against the p21 transforming protein encoded by the v-ras and c-ras (ras) oncogenes

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Y13-259细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with syngeneic normal rat kidney cells (NRK) that had been infected with Harvey murine sarcoma virus.

Spleen cells were fused with 210RCY3 Ag 1.2.3 myeloma cells.

The antibody reacts with both the Harvey (Ha-ras) and Kirsten (Ki-ras) p21 proteins.

The antibody also precipitates an endogenous p21 from a variety of rodent, carnivore and primate cell lines.

Propagation: Y13-259细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1320: Furth ME, et al. Monoclonal antibodies to the p21 products of the transforming gene of Harvey murine sarcoma virus and of the cellular ras gene family. J. Virol. 43: 294-304, 1982. Y13-259细胞PubMed: 6287003