Y-3细胞
细胞形态： **样
**类型： IgG2b; kappa light chain
生长状态： 悬浮生长
ATCC Number： HB-176™
是否是肿瘤细胞： 0
物种来源： 小鼠
数量： 大量
运输方式： 冻存运输
Designations: Y-3
Y-3细胞Depositors: C Janeway
Isotype: IgG2b; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against H-2 K b
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Comments: Animals were immunized with Con A stimulated spleen cells from BALB.B mice.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody cross-reacts with the k, p, q, r and s H-2 haplotypes.
Tested and found negative for ectromelia virus (mousepox).
Y-3细胞Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1308: Hammerling GJ, et al. Localization of allodeterminants on H-2Kb antigens determined with monoclonal antibodies and H-2 mutant mice. Proc. Natl. Acad. Sci. USA 79: 4737-4741, 1982. Y-3细胞PubMed: 6181513
