Lym-2细胞
ATCC Number： HB-8613™
数量： 大量
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 小鼠
生长状态： 悬浮生长
细胞形态： **样
**类型： IgG1
Designations: Lym-2
Lym-2细胞Depositors: Northwestern University
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against chronic lymphocytic leukemia (CLL) cells
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Lym-2细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with CLL cells.
Spleen cells were fused with NS-1 myeloma cells.
The antibody stains a majority of human B cell derived tumors, but does not react with solid tumors.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Lym-2细胞Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 3902: Epstein AL. Lym-2细胞Murine hybridoma Lym-2 and diagnostic antibody produced thereby. US Patent 4,724,212 dated Feb 9 1988
