TS2/9.1.4.3 [TS2/9.1.1.4.3]细胞
运输方式： 冻存运输
器官来源： 脾
细胞形态： **样
**类型： mouse IgG1
数量： 大量
生长状态： 悬浮生长
ATCC Number： HB-205™
是否是肿瘤细胞： 0
物种来源： 小鼠
TS2/9.1.4.3 [TS2/9.1.1.4.3]细胞Designations: TS2/9.1.4.3 [TS2/9.1.1.4.3]
Depositors: TA Springer
Isotype: mouse IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Organ: spleen
Cell Type: TS2/9.1.4.3 [TS2/9.1.1.4.3]细胞hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against lymphocyte function antigen 3 (LFA-3); against CD58
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: Please acknowledge the origin of this cell line in all relevant publications by citing the following publication(s): [1616]
Comments: Animals were immunized with human cytolytic T lymphocytes.
Spleen cells were fused with NS-1 myeloma cells.
The antibody will inhibit HLA DR mediated T cell cytotoxicity.
TS2/9.1.4.3 [TS2/9.1.1.4.3]细胞The depositor states that the cell line should not be maintained in culture continuously for greater than four months or antibody production may be lost.
Cloning and freezing of the cells after minimal passage (with selection of positive clones) will prevent loss of antibody producing cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml
Medium Renewal: TS2/9.1.4.3 [TS2/9.1.1.4.3]细胞Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1616: Sanchez-Madrid F, et al. Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3. Proc. Natl. Acad. Sci. USA 79: 7489-7493, 1982. PubMed: 6984191
