2F.11.15细胞

2F.11.15细胞

是否是肿瘤细胞： 0

物种来源： 小鼠

ATCC Number： TIB-194™

生长状态： 悬浮生长

细胞形态： **样

**类型： IgA

数量： 大量

运输方式： 冻存运输

2F.11.15细胞Designations: 2F.11.15

Depositors: H Nariuchi

Isotype: IgA

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: 2F.11.15细胞immunoglobulin; monoclonal antibody; against 2,4,6-trinitrophenyl (TNP)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with TNP-KLH.

2F.11.15细胞Spleen cells were fused with P3X63Ag8.653 myeloma cells.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. 2F.11.15细胞Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.