TS1/18.1.2.11细胞
是否是肿瘤细胞： 0
物种来源： 小鼠
生长状态： 悬浮生长
器官来源： 脾
ATCC Number： HB-203™
运输方式： 冻存运输
细胞形态： **样
**类型： mouse IgG1
数量： 大量
Designations: TS1/18.1.2.11
TS1/18.1.2.11细胞Depositors: TA Springer
Isotype: mouse IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Organ: spleen
Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against lymphocyte function antigen 1 (LFA-1) beta subunit; against CD18
Permits/Forms: TS1/18.1.2.11细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: Please acknowledge the origin of this cell line in all relevant publications by citing the following publication(s): [1616]
Comments: Animals were immunized with human cytolytic T lymphocytes.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The beta subunit of LFA-1 is a 95000 dalton polypeptide that is identical to the beta subunits of Mac-1 and the p150,95 antigens.
The antibody will inhibit HLA DR mediated T cell cytotoxicity.
The depositor states that the cell line should not be maintained in culture continuously for greater than four months or antibody production may be lost.
Cloning and freezing of the cells after minimal passage (with selection of positive clones) will prevent loss of antibody producing cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: TS1/18.1.2.11细胞ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(5) viable cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 1616: Sanchez-Madrid F, et al. Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3. Proc. Natl. Acad. Sci. USA 79: 7489-7493, 1982. PubMed: 6984191
32897: Zhang L, Plow EF. TS1/18.1.2.11细胞Overlapping, but not identical, sites are involved in the recognition of C3bi, neutrophil inhibitory factor, and adhesvie ligands by the alpha M beta 2 integrin. J. Biol. Chem. 271: 18211-18216, 1996. PubMed: 8663418
