28S.3细胞
细胞类型： 其他细胞类型
是否是肿瘤细胞： 0
物种来源： 其他
生长状态： 悬浮生长
ATCC Number： CRL-2758™
细胞形态： **样
运输方式： 冻存运输
数量： 大量
器官来源： 外周血
Designations: 28S.3
28S.3细胞Depositors: NW Miller
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Ictalurus punctatus
Morphology: lymphoblast
Source: Organ: peripheral blood
Cell Type: lymphocyte T lymphoblast;
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Isolation:28S.3细胞 Isolation date: 1996
Cytogenetic Analysis: diploid
Comments: The 28S.3 cell line, developed in 1996, is an immortal T cell line spontaneously derived from a long term culture of peripheral blood from a normal channel catfish. The cells were subsequently cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors. The cells are immunoglobulin negative [PubMed: 9809582].The cells constitutively produce a supernatant factor(s) with growth promoting activity [PubMed: 9809582].
Propagation: ATCC complete growth medium: A 1:1 mixture of Leibovitz's L-15 medium with 2 mM L-glutamine, and AIM-V Medium adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 87.5%; double distilled water, 10%; heat-inactivated Catfish serum, 2.5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
28S.3细胞Temperature: 27.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(5) viable cells/ml.
Interval: Maintain cell density between 5 X 10(5) and 5 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
References: 61272: Wilson MR, et al. 28S.3细胞T-cell receptors in channel catfish: structure and expression of TCR alpha and beta genes. Mol. Immunol. 35: 545-557, 1998. PubMed: 9809582
