Lu Vin细胞

Lu Vin细胞

年限： 6 years

器官来源： 皮肤

生长状态： 贴壁生长

运输方式： 冻存运输

细胞形态： 成纤维样

细胞类型： 成纤维细胞

是否是肿瘤细胞： 0

物种来源： 人

数量： 大量

ATCC Number： CRL-1144™

相关**： Ehlers-Danlos综合征

Lu Vin细胞Designations: Lu Vin

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: skin

Disease: Ehlers-Danlos syndrome, type I (autosomal dominant type)

Cell Type: fibroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Lu Vin细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Age: 6 years

Gender: female

Comments: The tissue donor suffered from Ehlers-Danlos syndrome (autosomal dominant type), and was the daughter of Mar Vin (ATCC CRL-1138) and the niece of Ron Har (ATCC CRL-1131).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. Lu Vin细胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Remove spent medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Lu Vin细胞Subculture every 7 to 9 days.