TR 310细胞

TR 310细胞

生长状态： 悬浮生长

ATCC Number： HB-219™

运输方式： 冻存运输

数量： 大量

是否是肿瘤细胞： 0

物种来源： 大鼠

细胞形态： **样

**类型： IgG2b

Designations: TR 310

TR 310细胞Depositors: IL Weissman

Isotype: IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: TR 310细胞immunoglobulin; monoclonal antibody; against mouse T cell receptor

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Comments: Animals were immunized with the Tk1 murine T cell lymphoma.

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

TR 310细胞The antibody reacts with a deteminant on the mouse T cell antigen receptor.

It recognizes a determinant encoded by the Vb7 (V beta 7) gene segment.

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine, 1 mM sodium pyruvate and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. TR 310细胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.