GeLu细胞

GeLu细胞

器官来源： 肺

细胞形态： 成纤维样

数量： 大量

运输方式： 冻存运输

生长状态： 贴壁生长

年限： 403 days

是否是肿瘤细胞： 0

物种来源： 其他

GeLu细胞ATCC Number： CCL-100™

Designations: GeLu

Depositors: ML Macy

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Meriones unguiculatus

Morphology: fibroblast

Source: Organ: lung

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. GeLu细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Virus Resistance: poliovirus 1, 3

Age: 403 days

Gender: female

Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, rinse cell sheet twice with 0.25% trypsin - 0.53 mM EDTA solution and remove. Place culture at room temperature until cells detach (approximately 10 to 20 minutes). Add fresh medium, aspirate and dispense into new flasks.

GeLu细胞Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

References: 26276: . . TCA GeLu细胞(Tissue Cult. Assoc.) Man. 3: 617-621, 1977.