2B5.3细胞
数量： 大量
是否是肿瘤细胞： 0
物种来源： 小鼠
生长状态： 悬浮生长
运输方式： 冻存运输
细胞形态： **样
**类型： IgG2b; kappa light chain
ATCC Number： CRL-1960™
Designations: 2B5.3
Depositors: MF Heyworth
Isotype: IgG2b; kappa light chain
2B5.3细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against Giardia muris trophozoites
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Comments: Animals were immunized with G. muris trophozoites.
Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells.
2B5.3细胞The antibody reacts with G. muris trophozoite surfaces.
Unlike 3C7.2 (ATCC CRL-1959), 1A3.1 (ATCC CRL-1961) and 1B1.1, the 2B5.3 antibody specifically recognizes trophozoite antigens of apparent molecular weight 31000 and 35000 on western blots.
The antibody immunoprecipitates two polypeptides with apparent molecular weights of 30000.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%;NCTC 109 medium, 10%;(Add 1 mM oxaloacetic acid, 0.45 mM sodium pyruvate, 4 mM L-glutamine, 0.1 mM hypoxanthine, 0.01 mM thymidine and 10 units/ml bovine insulin to this mixture);horse serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22728: Heyworth MF, et al. 2B5.3细胞Generation and characterization of monoclonal antibodies against Giardia muris trophozoites. Immunology 68: 341-345, 1989. PubMed: 2592009
