2BD4E4 K99细胞
运输方式： 冻存运输
ATCC Number： HB-8178™
细胞形态： **样
是否是肿瘤细胞： 0
物种来源： 小鼠
**类型： IgG1; kappa light chain
生长状态： 悬浮生长
数量： 大量
2BD4E4 K99细胞Designations: 2BD4E4 K99
Depositors: Molecular Genetics, Inc.
Isotype: IgG1; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: 2BD4E4 K99细胞hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against Escherichia coli K99 pilus
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Comments: Animals were immunized with E. coli K99 pilus.
Spleen cells were fused with NS-1 myeloma cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 15%
2BD4E4 K99细胞Temperature: 37.0°C
Subculturing: Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1005: Chan R, et al. Use of specific antibody to demonstrate glycocalyx, K99 pili and the spatial relationships of K99 & enterotoxigenic Escherichia coli in the ileum of colostrum-fed calves. Infect. Immun. 37: 1170-1180, 1982. PubMed: 6127313
3513: Sadowski PL. 2BD4E4 K99细胞Production of monoclonal antibodies against bacterial adhesins. US Patent 4,443,549 dated Apr 17 1984
