TK#1 [Embryonic stem cell line]细胞
运输方式： 冻存运输
生长状态： 贴壁生长
数量： 大量
细胞类型： 胚胎干细胞
是否是肿瘤细胞： 0
物种来源： 小鼠
器官来源： 胚胎
年限： embryo
TK#1 [Embryonic stem cell line]细胞细胞形态： 上皮样
ATCC Number： CRL-11383™
Designations: TK#1 [Embryonic stem cell line]
Depositors: TW Mak
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus deposited as mouse
Morphology: epithelial
Source: Organ: TK#1 [Embryonic stem cell line]细胞embryo
Cell Type: embryonic stem cell;
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Applications: The cell line was used to produce mutant mice with germ line transmission for the IRF-2 disruption.
Age: embryo
Comments: The construct pMIRF2neoB was introduced into D3 embryonic stem (ES) cells by electroporation to disrupt the IRF-2 locus.
The cell line generated is deficient for the interferon regulatory factor 2 (IRF-2) gene.
The cell line was used to produce mutant mice with germ line transmission for the IRF-2 disruption.
Heterozygous mice were inbred to obtain mice homozygous for the disrupted gene.
TK#1 [Embryonic stem cell line]细胞The line should be grown on feeder layers of irradiated (3000 rads) or mitomycin C treated (0.01 mg/ml for 90 minutes) primary mouse embryonic fibroblasts or STO cells (see ATCC CRL-1503 or ATCC 56-X, irradiated STO cells).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 mM 2-mercaptoethanol, 85%; fetal bovine serum, 15%
Temperature: 37.0°C
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
feeder layer cells:ATCC 56-X
References: 46928: TK#1 [Embryonic stem cell line]细胞Mak TW, Taniguchi T. Mouse lacking the expression of interferon regulatory factor 2 (IRF-2). US Patent 5,675,059 dated Oct 7 1997
