2A细胞
生长状态： 贴壁生长
数量： 大量
是否是肿瘤细胞： 0
物种来源： 人
ATCC Number： CRL-12013™
年限： fetus
器官来源： 肾脏
运输方式： 冻存运输
Designations: 2A
2A细胞Depositors: Chiron Viagene, Inc.
Biosafety Level: 2 [Cells contain adenoviral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens deposited as human
Morphology:
Source: Organ: kidney
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Age: fetus
Comments: 2A细胞The 2A amphotropic retroviral packaging cell line was derived from the human embryonic kidney line, 293 (see ATCC CRL-1573).
293 cells were co-transfected with the methotrexate resistance vector, pFR400 and the MoMLV gag/pol expression vector, pSCV10. [57428]
After selection for transfected cells, individual drug resistant cell colonies were expanded, analyzed and selected for overexpression of MoMLV gag/pol. [57428]
293 (clone 2-3) was co-transfected with the phleomycin resistance vector, pUT507 and the amphotropic envelope expression vector, pMLPenvAmSph and subsequently cloned. [57428]
The 2A clone was selected for expression of relatively high levels of both gag/pol and amphotropic envelope proteins. [57428]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
O.1 mM Non-Essential Amino Acids (NEAA)
fetal bovine serum to a final concentration of 10%
2A细胞Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
parental cell line:ATCC CRL-1573
References: 43969: DePolo NJ, et al. 2A细胞The resistance of retroviral vectors produced from human cells to serum inactivation in vivo and in vitro is primate species dependent. J. Virol. 73: 6708-6714, 1999. PubMed: 10400768
57428: Barber JR, et al. Retroviral packaging cell lines. US Patent 5,591,624 dated Jan 7 1997
