TK-1细胞
细胞形态： **样
细胞类型： T**细胞
是否是肿瘤细胞： 0
物种来源： 小鼠
品系： AKR/Cum
生长状态： 悬浮生长
数量： 大量
ATCC Number： CRL-2396™
运输方式： 冻存运输
相关**： **瘤
Designations: TK-1
Depositors: EC Butcher
TK-1细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus deposited as mouse
Morphology: lymphoblast
Source: Strain: AKR/Cum
Disease: lymphoma
Cell Type: T lymphocyte;
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Antigen Expression: CD8 +; alpha4, beta7 integrin +
Comments: TK-1细胞TK1 is a T-cell lymphoma cell line originated by E. C. Butcher and I. L. Weissman from a spontaneous thymic lymphoma/leukemia that developed in an AKR/Cum mouse. [38634]
TK1 cells have a unique phenotype with high levels of the lymphocyte integrin, alpha 4, beta 7.
They bind constitutively to the murine mucosal vascular addressin, MAdCAM-1. [22636]
TK1 cells are useful for studies of alpha 4, beta 7 function and regulation. The cells are CD8 positive; they display approximately 1% of L-selectin of that expressed by normal lymph node cells.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.1 mM non-essential amino acids, 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 1 to 2 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 2 X 10(6) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: TK-1细胞Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22636: Berlin C, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993. PubMed: 7687523
38633: Berlin C, et al. alpha 4 integrins mediate lymphocyte attachment and rolling under physiologic flow. Cell 80: 413-422, 1995. PubMed: 7532110
38634: Butcher EC, et al. Organ specificity of lymphocyte migration: mediation by highly selective lymphocyte interaction with organ-specific determinants on high endothelial venules. Eur. J. Immunol. 10: 556-561, 1980. TK-1细胞PubMed: 6157544
