LP4.4细胞
数量： 大量
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 小鼠
生长状态： 悬浮生长
ATCC Number： HB-232™
细胞形态： **样
**类型： IgG2b; kappa light chain
LP4.4细胞Designations: LP4.4
Depositors: H Kubagawa, JF Kearney
Isotype: IgG2b; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:LP4.4细胞 immunoglobulin; monoclonal antibody; against human IgM, lambda
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Comments: Animals were immunized with a human IgM, kappa paraprotein (LP) which reacted with intermediate filaments.
Lymph node cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with most human IgM, lambda and some human IgM, kappa molecules.
It appears to react with a conformational determinant expressed preferentially on IgM, lambda complexes.
LP4.4细胞It also reacts with mu - surrogate light chain complexes, and provides a marker for pre-B and early B cell differentiation.
It does not react with isolated mu, lambda or kappa chains.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22490: Ohno T, et al. LP4.4细胞A mouse monoclonal antibody reactive preferentially with human IgM lambda. Hybridoma 9: 473-480, 1990. PubMed: 1701760
