129A3/1细胞

129A3/1细胞

数量： 大量

运输方式： 冻存运输

是否是肿瘤细胞： 0

物种来源： 小鼠

生长状态： 悬浮生长

细胞形态： **样

**类型： IgA

ATCC Number： HB-8087™

Designations: 129A3/1

Depositors: Johns Hopkins University

Isotype: IgA

129A3/1细胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against surface (cercariae) glycoprotein on Schistosoma mansoni

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. 129A3/1细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with cercariae glycoprotein from Schistosoma mansoni.

Spleen cells were fused with P3X63Ag8 myeloma cells.

Propagation: ATCC complete growth medium: Modified Dulbecco's medium with 0.1 mM hypoxanthine, 400 nM aminopterin and 0.016 mM thymidine, 90%; fetal bovine serum, 10%

129A3/1细胞Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: recommended serum:ATCC 30-2020

References: 3511: Strand M. Diagnosis and treatment of fluke infections with monoclonal antibodies. 129A3/1细胞US Patent 4,416,866 dated Nov 22 1983