G1B细胞

G1B细胞

运输方式： 冻存运输

生长状态： 贴壁生长

器官来源： 其他

是否是肿瘤细胞： 0

物种来源： 其他

数量： 大量

细胞形态： 其他

ATCC Number： CRL-2536™

年限： *****

Designations: G1B

G1B细胞Depositors: EJ Noga

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Clarias batrachus

Morphology: pleomorphic

Source: Organ: gill

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Age: *****

Comments: G1B细胞The G1B cell line was derived from normal gill tissue taken from a healthy ***** walking catfish. [49660]

The cells support the in vitro growth of Amyloodinium ocellatum, a dinoflagellate that infects the gills and skin of both marine and brackish water fishes.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 10%

24 mM HEPES

Temperature: 25.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin-0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. G1B细胞Allow the flask to sit at room temperature (or at 25.0°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.NOTE: Some batches of fetal bovine serum are toxic to these cells.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: Twice per week

Preservation: culture medium 95%; DMSO, 5%

References: 49660: Noga EJ, Hartmann JX. Establishment of walking catfish (Clarias batrachus) cell lines and development of a channel catfish (Ictalurus punctatus) virus vaccine. Can. J. Fish. G1B细胞Aquat. Sci. 38: 925-930, 1981.