G-8细胞

G-8细胞

生长状态： 贴壁生长

组织来源： skeletal muscle

细胞形态： 成肌细胞

年限： fetus fetus

运输方式： 冻存运输

细胞类型： 其他细胞类型

是否是肿瘤细胞： 0

G-8细胞物种来源： 小鼠

ATCC Number： CRL-1456™

数量： 大量

Designations: G-8

Depositors: J Peacock

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

G-8细胞Morphology: myoblast

Source: Tissue: skeletal muscle

Cell Type: myoblast myoblast;

Cellular Products: myosin

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Receptors: acetylcholine (The cells are highly responsive to acetylcholine.)

Tumorigenic: No

G-8细胞Cytogenetic Analysis: modal number = 66

Age: fetus fetus

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53mM EDTA solution for 2 to 5 minutes, add fresh medium, aspirate and dispense into new Bovine Collagen type l (0.03 mg/ml) coated flasks.Differentiation is enhanced by reducing the sera to 2% each.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Twice per week

Doubling Time: 25 to 27 hrs

References: 1063: Christian CN, et al. G-8细胞Synapse formation between two clonal cell lines. Science 196: 995-998, 1977. PubMed: 193191