LLC-WRC 256细胞

LLC-WRC 256细胞

运输方式： 冻存运输

是否是肿瘤细胞： 0

物种来源： 褐鼠

生长状态： 贴壁生长

品系： Harlan-Wistar

ATCC Number： CCL-38™

相关**： 其他**

细胞形态： 上皮样

数量： 大量

LLC-WRC 256细胞Designations: LLC-WRC 256

Depositors: RN Hull

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Rattus norvegicus

Morphology: epithelial

Source: Disease: mammary gland; Walker carcinoma

Strain: Harlan-Wistar

Permits/Forms:LLC-WRC 256细胞 In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Virus Resistance: poliovirus 1

Tumorigenic: Yes

LLC-WRC 256细胞Propagation: ATCC complete growth medium: Medium 199, 95%; horse serum, 5%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

Medium Renewal: Twice per week

Remove medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.

References: 22310: . . Anat. Rec. 124: 490, 1956.

LLC-WRC 256细胞26193: . . Science 117: 223, 1953.