TF-1.CN5a.1细胞
年限： 35 years
运输方式： 冻存运输
细胞形态： **样
器官来源： 骨髓
ATCC Number： CRL-2512™
相关**： 白血病
细胞类型： 其他细胞类型
是否是肿瘤细胞： 0
物种来源： 人
数量： 大量
生长状态： 悬浮生长
TF-1.CN5a.1细胞Designations: TF-1.CN5a.1
Depositors: CJ Robinson
Biosafety Level: 2 [Cells contain CMV viral sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens deposited as human
Morphology: lymphoblast
Source: Organ: bone marrow
Disease: erythroleukemia
Cell Type: erythroblast
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Receptors: alpha subunit of human ciliary neurotrophic factor (CNTF) (receptor is not expressed by TF-1 cells)
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 13
D13S317: 8,9
D16S539: 9,12
D5S818: 13
D7S820: 12
THO1: 7,9
TPOX: 8
vWA: 15,17
Age: 35 years
Gender: male
Ethnicity: Japanese
Comments: The cells stably express the alpha subunit of human ciliary neurotrophic factor (CNTF) receptor and CNTF supports the short term proliferation of the cells. TF-1.CN5a.1细胞Suggested assay conditions are washing the cells with RPMI to remove GM-CSF, then incubation in RPMI 1640 95%, fetal bovine serum 5%, and picogram/ml concentrations of CNTF. The cells maintain responsiveness for at least 193 population doublings in full maintenance medium.
This cell line was derived from the TF-1 cell line (ATCC CRL-2003).
TF-1 cells were transfected, using Transfectam, with the gene for the alpha subunit of human ciliary neurotrophic factor (CNTF) receptor ligated to the pCR3.1 vector.
The vector contains cytomegalovirus (CMV) and SV40 viral sequences and the neomycin resistance gene.
Following transfection, a line of G418 resistant cells was obtained and named TF-1.CN5a.
TF-1.CN5a cells were grown, transiently, in the absence of GM-CSF and with 2 ng/ml CNTF to establish the TF-1.CN5a.1 cell line.
The cells can be used to access the potency and to study signal transduction of CNTF.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with 2 ng/ml GM-CSF and 0.4 mg/ml G-418, 90%; fetal bovine serum, 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing:TF-1.CN5a.1细胞Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
parental cell line:ATCC CRL-2003
