LL 24细胞

LL 24细胞

细胞类型： 成纤维细胞

是否是肿瘤细胞： 0

物种来源： 人

年限： 5 years

器官来源： 肺

运输方式： 冻存运输

ATCC Number： CCL-151™

相关**： 正常

细胞形态： 成纤维样

生长状态： 贴壁生长

数量： 大量

LL 24细胞Designations: LL 24

Depositors: RG Crystal

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: lung

Disease: normal

Cell Type: fibroblast

Permits/Forms:LL 24细胞 In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 9,12

D13S317: 8,11

D16S539: 9,12

D5S818: 10,11

D7S820: 11,12

THO1: 7

TPOX: 8

vWA: 16,18

Cytogenetic Analysis: normal human male; diploid; stable

Isoenzymes: G6PD, B

LL 24细胞Age: 5 years

Gender: male

Ethnicity: Caucasian

Propagation: ATCC complete growth medium: Ham's F12K medium, 85%; fetal bovine serum, 15% Other standard media (EMEM or McCoy's 5a) may be used

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, add fresh 0.25% trypsin, 0.03% EDTA for 1 to 2 minutes, remove most of the trypsin and incubate at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks.

References: 21405: Sugarman BJ, et al. LL 24细胞Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111